Bacteriophages: Methods and Protocols, Volume IV by Martha R.J. Clokie (English)-image

Bacteriophages: Methods and Protocols, Volume IV by Martha R.J. Clokie (English)

$ 223.88

ISBN-13: 9781493989393 ISBN: 9781493989393 Type: NA Publication Name: NA gtin13: 9781493989393

Description

The Nile on eBay FREE SHIPPING UK WIDE Bacteriophages by Martha R.J. Clokie, Andrew Kropinski, Rob Lavigne This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems'. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both establishedand novice phage scientists. Back Cover This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag Table of Contents PhageFISH for Monitoring Phage Infections at Single Cell Level.- Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria.- Engineering Bacteriophage-Based Biosensors.- Introduction of Phage Genome into Escherichia coli by Electroporation.- Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1.- Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA.- Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography.- Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes.- Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis.- Analyzing Phage-Host Protein-Protein Interactions using Strep-tag® II Purifications.- Techniques to Assess Phage/Biofilm Interaction.- Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages.- The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis.- Use of a Silkworm Larva Model in Phage Therapy Experiments.- Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages.- Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems.- Quantitating Phage Efficacy in ready-To-Eat Meats.- Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy. Review "The book describes the functional elucidation of bacteriophages in the current era, as well as the drive towards application-driven research. … this is a very interesting resource for protocols regarding phages and their use for therapy. I am going to include it in my reference material for my own students working on phages in relation to the gut microbiota." (Koen Venema, Beneficial Microbes, Vol. 10 (3), 2019) Review Quote "The book describes the functional elucidation of bacteriophages in the current era, as well as the drive towards application-driven research. ... this is a very interesting resource for protocols regarding phages and their use for therapy. I am going to include it in my reference material for my own students working on phages in relation to the gut microbiota." (Koen Venema, Beneficial Microbes, Vol. 10 (3), 2019) Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN 1493989391 Publisher Humana Press Inc. Series Methods in Molecular Biology ISBN-10 1493989391 ISBN-13 9781493989393 Format Hardcover Imprint Humana Press Inc. Subtitle Methods and Protocols, Volume IV Place of Publication Totowa, NJ Country of Publication United States Edited by Rob Lavigne DEWEY 579.26 Pages 216 Year 2018 Publication Date 2018-12-20 Short Title Bacteriophages Language English DOI 10.1007/978-1-4939-8940-9 Series Number 1898 UK Release Date 2018-12-20 AU Release Date 2018-12-20 NZ Release Date 2018-12-20 US Release Date 2018-12-20 Author Rob Lavigne Audience Professional & Vocational Illustrations 46 Illustrations, color; 35 Illustrations, black and white; XI, 216 p. 81 illus., 46 illus. in color. Edition Description 2019 ed. Edition 2019th Country of Origin NL Product Class Description Life Sciences: General We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 30 DAY RETURN POLICY No questions asked, 30 day returns! FREE DELIVERY No matter where you are in the UK, delivery is free. SECURE PAYMENT Peace of mind by paying through PayPal and eBay Buyer Protection TheNile_Item_ID:137806861;

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